Thank you for your suggestion. Because of the significant bioactivities of chintosan, it is necessary to detect the concentration of CTS for more effective and scientific application. When CTS interacted with BB, the RRS intensities were increased obviously, expecially at 344 nm and 452nm. Base on these, a simple, rapid and sensitive DWO-RRS method was established, which method and system for the determination of chitosan has not been reported in the literature. More importantly, we have fully consider the effect of the molecular weight of CTS and different degree of deacetylation of CTS. Ahout the second problem, we have revised the whole manuscript according to your suggestion on our revised manuscript.