Directions: Present your perspective on one of the issues below, using relevant reasons and/or examples to support your views. “The function of science is to reassure; the purpose of art is to upset. Therein lies the value of each.”
请使用灵活多样的词汇表达;能够很好的使用长难句且句法很棒;文中基本没有过渡词和衔接词,建议增加衔接词汇的积累;请注意分段;请提高文章内敛性,段落和句子要围绕主题展开;可以适当增加副词的使用。
The assay was carried out according to the method of Wardlaw and Willenbrink (1994) with slight modifications. The reaction mixture (110 µl) contained 50 mM Hepes-NaOH (pH 7.5), 100 mM fructose-6-phosphate(for SPS) or fructose(for SS), 100 mM UDPglucose, and 50 mM MgCl2. The reaction was started by adding 50 µl of crude enzyme extract. After incubating at 30C for 30 min, the reaction was stopped by boiling for 1 min. After adding 200 µl of 2 M NaOH, the solution was incubated for 10 min at 100C. After cooling, 2 ml of 30% (v/v) HCl and 1 ml of 0.1% (w/v) resorcin were added and the solution incubated for 10 min at 80C. Finally, sucrose content was determined by measuring the absorbance at 480 nm with a spectrophotometer.