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Therefore, we agreed the following reason, may be the sperm quality is completely poor in AZF patients,so it's difficult to select sperm satisfactory for ICSI, finally it will effects the embryonic development in early stage, such as in fertilization and cleavage stage, once successful fertilization and cleavage are achieved, embryonic development and clinical outcomes would not be affected by AZF microdeleitons, our results confirmed the results of previous studies in a larger cohort